|
KMID : 0617320010100010091
|
|
Journal of Pharmacetical Sceiences Ewha Womans University
2001 Volume.10 No. 1 p.91 ~ p.95
|
|
|
Identification of the Interaction between Rat Translationally Controlled Tumor Protein/IgE-dependent Histamine Releasing Factor and Myosin Light Chain
|
|
Kim, Min-Jeong
Jung, Jaehoon/Choi, Eung Chil/Park, Hae-Young/Lee, Kyunglim
|
|
|
Abstract
|
|
|
|
The translationally controlled tumor protein(TCTP), also known as the lgE-dependent histamine releasing factor(HRF), was used in the yeast tow-hybrid system to screen the interacting molecules. We obtained the N-terminus truncated rat fast myosin alkai light chain from the rat skeletal muscle cDNA library in the screening. Since either TCTP/HRF or the myosin light chain is known to be associated with histamine secretion from RBL-2H3 cells, we investigated the possible interaction between rat TCTP/HRF and nonmuscle myosin light chain in these cells. We used affinity chromatography and coimmunoprecipitation. Our data suggests that HRF and the myosin light chain interact, which may play an important role in histamine release in RBL-2H3 cells.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|